ATP5B recombinant monoclonal antibody, clone 5F10
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: 293T whole cell lysate; Lane 2: HT29 whole cell lysate; Lane 3: HepG2 whole cell lysate; Lane 4: Jurkat whole cell lysate; Lane 5: 293 whole cell lysate; Lane 6: Rat Heart tissue; Lane 7: Mouse Heart tissue.
Immunohistochemistry
Immunohistochemistry image of ATP5B recombinant monoclonal antibody, clone 5F10 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human, mouse and rat ATP5B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human ATP5B.
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: 293T whole cell lysate; Lane 2: HT29 whole cell lysate; Lane 3: HepG2 whole cell lysate; Lane 4: Jurkat whole cell lysate; Lane 5: 293 whole cell lysate; Lane 6: Rat Heart tissue; Lane 7: Mouse Heart tissue.Immunohistochemistry
Immunohistochemistry image of ATP5B recombinant monoclonal antibody, clone 5F10 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATP5B
Entrez GeneID
506Protein Accession#
P06576Gene Name
ATP5B
Gene Alias
ATPMB, ATPSB, MGC5231
Gene Description
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Omim ID
102910Gene Ontology
HyperlinkGene Summary
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq
Other Designations
ATP synthase, H+ transporting, mitochondrial F1 complex, beta subunit|mitochondrial ATP synthetase, beta subunit
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Interactome
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Pathway
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Disease
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