G3BP1 recombinant monoclonal antibody, clone R09-5B9
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More Files
- More Functions
Western Blot
Western blot analysis of PLK1 in Jurkat, C6 lysates using PLK1 antibody.
Western Blot
Western blot analysis of G3BP in K562, C6, Hela lysates using G3BP antibody.
Immunocytochemistry
Immunocytochemistry analysis of PLK1 (green) in Hela using PLK1 antibody,and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human G3BP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human G3BP1.
Theoretical MW (kDa)
Calculated MW: 52 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of PLK1 in Jurkat, C6 lysates using PLK1 antibody.Western Blot
Western blot analysis of G3BP in K562, C6, Hela lysates using G3BP antibody.Immunocytochemistry
Immunocytochemistry analysis of PLK1 (green) in Hela using PLK1 antibody,and DAPI(blue).Immunocytochemistry
Immunofluorescence
Immunofluorescence
Immunoprecipitation
Immunoprecipitation
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Gene Info — G3BP1
Entrez GeneID
10146Protein Accession#
Q13283Gene Name
G3BP1
Gene Alias
G3BP, HDH-VIII, MGC111040
Gene Description
GTPase activating protein (SH3 domain) binding protein 1
Omim ID
608431Gene Ontology
HyperlinkGene Summary
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
Other Designations
GAP binding protein|OTTHUMP00000160618|Ras-GTPase-activating protein SH3-domain-binding protein|RasGAP-associated endoribonuclease G3BP
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Interactome
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