G3BP1 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
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Western Blot (Transfected lysate)
Western Blot analysis of G3BP1 expression in transfected 293T cell line (H00010146-T02) by G3BP1 MaxPab polyclonal antibody.
Lane 1: G3BP1 transfected lysate(52.20 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to G3BP1 on 293T cell. [antibody concentration 1 ug/ml]
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to G3BP1 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human G3BP1 protein.
Immunogen
G3BP1 (NP_005745.1, 1 a.a. ~ 466 a.a) full-length human protein.
Sequence
MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of G3BP1 expression in transfected 293T cell line (H00010146-T02) by G3BP1 MaxPab polyclonal antibody.
Lane 1: G3BP1 transfected lysate(52.20 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to G3BP1 on 293T cell. [antibody concentration 1 ug/ml]Immunofluorescence
Immunofluorescence of purified MaxPab antibody to G3BP1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — G3BP1
Entrez GeneID
10146GeneBank Accession#
NM_005754Protein Accession#
NP_005745.1Gene Name
G3BP1
Gene Alias
G3BP, HDH-VIII, MGC111040
Gene Description
GTPase activating protein (SH3 domain) binding protein 1
Omim ID
608431Gene Ontology
HyperlinkGene Summary
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
Other Designations
GAP binding protein|OTTHUMP00000160618|Ras-GTPase-activating protein SH3-domain-binding protein|RasGAP-associated endoribonuclease G3BP
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Interactome
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Publication Reference
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Ataxin-2-like is a regulator of stress granules and processing bodies.
Kaehler C, Isensee J, Nonhoff U, Terrey M, Hucho T, Lehrach H, Krobitsch S.
PLoS One 2012 Nov; 7(11):e50134.
Application：IF, IP-WB, WB-Ce, Human, HEK 293T, HeLa cells.
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Ataxin-2-like is a regulator of stress granules and processing bodies.
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