G3BP 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-G3BP full-length
Host
Human
Theoretical MW (kDa)
52.2
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-G3BP antibody (H00010146-B01P) by Western Blots.
SDS-PAGE Gel
G3BP1 transfected lysate.
Western Blot
Lane 1: G3BP1 transfected lysate ( 52.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — G3BP1
Entrez GeneID
10146GeneBank Accession#
NM_005754Protein Accession#
NP_005745.1Gene Name
G3BP1
Gene Alias
G3BP, HDH-VIII, MGC111040
Gene Description
GTPase activating protein (SH3 domain) binding protein 1
Omim ID
608431Gene Ontology
HyperlinkGene Summary
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq
Other Designations
GAP binding protein|OTTHUMP00000160618|Ras-GTPase-activating protein SH3-domain-binding protein|RasGAP-associated endoribonuclease G3BP
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