TNFRSF10B recombinant monoclonal antibody, clone 304
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More Files
- More Functions
Western Blot
Western Blot analysis were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 304 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric rabbit IgG version of 304 (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.
Flow Cytometry
Flow-cytometry were stained with an isotype control (black line) or the rabbit-chimeric version of 304 (blue line) at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488 conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human DR5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against in mice homozygous for both disrupted endogenous Ig heavy chain and k light chain, harboring a chromosome 14 fragment (SC20) containing a human Ig heavy chain locus and a human Igk chain transgene (KCo5). The mice were immunised against TRAIL-R1 and TRAIL-R2.
Reactivity
Human
Specificity
The antibody is specific to human DR5.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG, Kappa
Recommend Usage
Flow cytometry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Applications
Western Blot
Western Blot analysis were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 304 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric rabbit IgG version of 304 (1:1000 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody followed by staining with Alexa Fluor 488 secondary antibody.Flow Cytometry
Flow-cytometry were stained with an isotype control (black line) or the rabbit-chimeric version of 304 (blue line) at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488 conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer. -
Gene Info — TNFRSF10B
Entrez GeneID
8795Protein Accession#
O14763Gene Name
TNFRSF10B
Gene Alias
CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9
Gene Description
tumor necrosis factor receptor superfamily, member 10b
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq
Other Designations
Fas-like protein|OTTHUMP00000123492|TNF-related apoptosis-inducing ligand receptor 2|TRAIL receptor 2|apoptosis inducing protein TRICK2A/2B|apoptosis inducing receptor TRAIL-R2|cytotoxic TRAIL receptor-2|death domain containing receptor for TRAIL/Apo-2L|d
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Interactome
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Pathway
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Disease
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