MAP1LC3A (phospho S12) polyclonal antibody

Catalog # PAB8066

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Size:400 uL
Price: USD $ 346.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Immunoblots of Phospho-MAP1LC3A in CHO cell culture. MAP1LC3 and MAP1LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with MAP1LC3A (phospho S12) polyclonal antibody (Cat # PAB8066). MAP1LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of MAP1LC3 with alanine. WT = wildtype MAP1LC3-transfected cell lysates; S12A = MAP1LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no MAP1LC3 gene. Molecular size: MAP1LC3-I = 16 KDa, and MAP1LC3-II = 14 KDa.

Dot Blot (Peptide)
Application

Dot Blot (Peptide)

Dot blot analysis of MAP1LC3A (phospho S12) polyclonal antibody (Cat # PAB8066) and Nonphospho-MAP1LC3 antibody on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

  • Specification

    Product Description

    Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP1LC3A.

    Immunogen

    Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S12 of human MAP1LC3A.

    Host

    Rabbit

    Reactivity

    Human

    Form

    Liquid

    Purification

    Protein A purification

    Recommend Usage

    ELISA (1:1000)
    Dot Blot (1:100-500)
    Western Blot (1:100-500)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Immunoblots of Phospho-MAP1LC3A in CHO cell culture. MAP1LC3 and MAP1LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with MAP1LC3A (phospho S12) polyclonal antibody (Cat # PAB8066). MAP1LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of MAP1LC3 with alanine. WT = wildtype MAP1LC3-transfected cell lysates; S12A = MAP1LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no MAP1LC3 gene. Molecular size: MAP1LC3-I = 16 KDa, and MAP1LC3-II = 14 KDa.

    Enzyme-linked Immunoabsorbent Assay

    Dot Blot (Peptide)

    Dot blot analysis of MAP1LC3A (phospho S12) polyclonal antibody (Cat # PAB8066) and Nonphospho-MAP1LC3 antibody on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
  • Gene Info — MAP1LC3A

    Entrez GeneID

    84557

    Protein Accession#

    NP_115903;Q5JWU0

    Gene Name

    MAP1LC3A

    Gene Alias

    LC3, LC3A, MAP1ALC3, MAP1BLC3

    Gene Description

    microtubule-associated protein 1 light chain 3 alpha

    Omim ID

    601242

    Gene Ontology

    Hyperlink

    Gene Summary

    MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

    Other Designations

    MAP1 light chain 3-like protein 1|MAP1A/1B light chain 3 A|MAP1A/MAP1B LC3 A|OTTHUMP00000030696|OTTHUMP00000030697|OTTHUMP00000030698|microtubule-associated proteins 1A/1B light chain 3

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  • Publication Reference
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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