CBX3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
CBX3 polyclonal antibody (Cat # PAB6885) (0.3 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CBX3 polyclonal antibody (Cat # PAB6885) (2.5 ug/mL) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CBX3.
Immunogen
A synthetic peptide corresponding to human CBX3.
Sequence
C-EAFLNSQKAGKEKD
Host
Goat
Theoretical MW (kDa)
20.8
Reactivity
Human, Mouse
Specificity
This antibody is not expected to cross-react with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.3-1.0 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
CBX3 polyclonal antibody (Cat # PAB6885) (0.3 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CBX3 polyclonal antibody (Cat # PAB6885) (2.5 ug/mL) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CBX3
Entrez GeneID
11335Protein Accession#
NP_009207.2;NP_057671.2Gene Name
CBX3
Gene Alias
HECH, HP1-GAMMA, HP1Hs-gamma
Gene Description
chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Omim ID
604477Gene Ontology
HyperlinkGene Summary
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. [provided by RefSeq
Other Designations
HP1 gamma homolog|OTTHUMP00000024529|OTTHUMP00000122519|chromobox homolog 3|heterochromatin protein HP1 gamma|heterochromatin-like protein 1
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Interactome
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Publication Reference
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Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin.
Vakoc CR, Mandat SA, Olenchock BA, Blobel GA.
Molecular Cell 2005 Aug; 19(3):381.
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Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin.
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