KPNA4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
KPNA4 polyclonal antibody (Cat # PAB6561) staining (0.1 ug/mL) of Human Ovary (1), Mouse Testes (2), (0.3 ug/mL) Mouse Overy (3) and (0.03 ug/mL) Rat Testes (4) lysate (RIPA buffer, 35 ug total protein per lane). Detected by chemiluminescence.
Western Blot (Tissue lysate)
KPNA4 polyclonal antibody (Cat # PAB6561) staining (0.5 ug/mL) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of KPNA4.
Immunogen
A synthetic peptide corresponding to C-terminus of human KPNA4.
Sequence
C-NSSANVPTEGFQF
Host
Goat
Theoretical MW (kDa)
58
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.03-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
KPNA4 polyclonal antibody (Cat # PAB6561) staining (0.1 ug/mL) of Human Ovary (1), Mouse Testes (2), (0.3 ug/mL) Mouse Overy (3) and (0.03 ug/mL) Rat Testes (4) lysate (RIPA buffer, 35 ug total protein per lane). Detected by chemiluminescence.Western Blot (Tissue lysate)
KPNA4 polyclonal antibody (Cat # PAB6561) staining (0.5 ug/mL) of human testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KPNA4
Entrez GeneID
3840Protein Accession#
NP_002259.1Gene Name
KPNA4
Gene Alias
IPOA3, MGC12217, MGC26703, QIP1, SRP3
Gene Description
karyopherin alpha 4 (importin alpha 3)
Omim ID
602970Gene Ontology
HyperlinkGene Summary
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq
Other Designations
importin alpha 3|importin-alpha-Q1|karyopherin alpha 4
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Interactome
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Publication Reference
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Cutting Edge: Tubulin {alpha} Functions as an Adaptor in NFAT-Importin {beta} Interaction.
Ishiguro K, Ando T, Maeda O, Watanabe O, Goto H.
The Journal of Immunology 2011 Mar; 186(5):2710.
Application：WB, Human, Jurkat cells.
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Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL.
Seki T, Tada S, Katada T, Enomoto T.
Biochemical and Biophysical Research Communications 1997 May; 234(1):48.
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Cutting Edge: Tubulin {alpha} Functions as an Adaptor in NFAT-Importin {beta} Interaction.
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