FOXO4 (phospho S197) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293 cells, Lane 2: serum treated 293 cells with FOXO4 (phospho S197) polyclonal antibody (Cat# PAB29614) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by FOXO4 (phospho S197) polyclonal antibody (Cat# PAB29614) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescent staining of methanol-fixed MCF7 cells using FOXO4 (phospho S197) polyclonal antibody (Cat# PAB29614) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human FOXO4.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S197 of human FOXO4.
Host
Rabbit
Theoretical MW (kDa)
65
Reactivity
Human, Mouse, Rabbit
Specificity
FOXO4 (phospho S197) polyclonal antibody detects endogenous level FOXO4 only when phosphorylated at serine 197.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: 293 cells, Lane 2: serum treated 293 cells with FOXO4 (phospho S197) polyclonal antibody (Cat# PAB29614) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue by FOXO4 (phospho S197) polyclonal antibody (Cat# PAB29614) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescent staining of methanol-fixed MCF7 cells using FOXO4 (phospho S197) polyclonal antibody (Cat# PAB29614) at 1:100-1:200 dilution. -
Gene Info — FOXO4
Entrez GeneID
4303Protein Accession#
P98177Gene Name
FOXO4
Gene Alias
AFX, AFX1, MGC120490, MLLT7
Gene Description
forkhead box O4
Omim ID
300033Gene Ontology
HyperlinkGene Summary
7|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog
Other Designations
OTTHUMP00000023496|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 7|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
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Interactome
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Disease
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