FOXO4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FOXO4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human FOXO4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse heart tissue lysate (35 ug/lane) with FOXO4 polyclonal antibody (Cat # PAB4778).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with FOXO4 polyclonal antibody (Cat # PAB4778) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — FOXO4
Entrez GeneID
4303Protein Accession#
NP_005929;P98177Gene Name
FOXO4
Gene Alias
AFX, AFX1, MGC120490, MLLT7
Gene Description
forkhead box O4
Omim ID
300033Gene Ontology
HyperlinkGene Summary
7|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog
Other Designations
OTTHUMP00000023496|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 7|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
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Interactome
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Disease
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Publication Reference
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Neutrophil activation by fMLP regulates FOXO (forkhead) transcription factors by multiple pathways, one of which includes the binding of FOXO to the survival factor Mcl-1.
Crossley LJ.
Journal of Leukocyte Biology 2003 Oct; 74(4):583.
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The forkhead transcription factor FOXO4 induces the down-regulation of hypoxia-inducible factor 1 alpha by a von Hippel-Lindau protein-independent mechanism.
Tang TT, Lasky LA.
The Journal of Biological Chemistry 2003 Aug; 278(32):30125.
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Neutrophil activation by fMLP regulates FOXO (forkhead) transcription factors by multiple pathways, one of which includes the binding of FOXO to the survival factor Mcl-1.
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