MAX (phospho S11) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated Jurkat cells, Lane 2: Starvation treated Jurkat cells with MAX (phospho S11) polyclonal antibody (Cat # PAB29594) at 1:500-1:2000 dilution.
Immunofluorescence
Immunofluorescent staining of U-2 OS cells with MAX (phospho S11) polyclonal antibody (Cat # PAB29594) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MAX.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S11 of human MAX.
Host
Rabbit
Theoretical MW (kDa)
21
Reactivity
Human
Specificity
MAX (phospho S11) polyclonal antibody detects endogenous levels of human MAX only when phosphorylated at serine 11.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated Jurkat cells, Lane 2: Starvation treated Jurkat cells with MAX (phospho S11) polyclonal antibody (Cat # PAB29594) at 1:500-1:2000 dilution.Immunofluorescence
Immunofluorescent staining of U-2 OS cells with MAX (phospho S11) polyclonal antibody (Cat # PAB29594) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining. -
Gene Info — MAX
Entrez GeneID
4149Protein Accession#
P61244Gene Name
MAX
Gene Alias
MGC10775, MGC11225, MGC18164, MGC34679, MGC36767, bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, orf1
Gene Description
MYC associated factor X
Omim ID
154950Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown. [provided by RefSeq
Other Designations
MAX protein|helix-loop-helix zipper protein|myc-associated factor X
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Interactome
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Pathway
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