WASF1 (phospho Y125) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Insulin (0.01 U/mL, 15 mins) treated NIH/3T3 cells, Lane 2: Synthesized peptide treated NIH/3T3 cells with WASF1 (phospho Y125) polyclonal antibody (Cat # PAB29590) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma tissue with WASF1 (phospho Y125) polyclonal antibody (Cat # PAB29590) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human WASF1.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y125 of human WASF1.
Host
Rabbit
Theoretical MW (kDa)
70
Reactivity
Human, Mouse
Specificity
WASF1 (phospho Y125) polyclonal antibody detects endogenous levels of human WASF1 only when phosphorylated at tyrosine 125.
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Insulin (0.01 U/mL, 15 mins) treated NIH/3T3 cells, Lane 2: Synthesized peptide treated NIH/3T3 cells with WASF1 (phospho Y125) polyclonal antibody (Cat # PAB29590) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon carcinoma tissue with WASF1 (phospho Y125) polyclonal antibody (Cat # PAB29590) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B). -
Gene Info — WASF1
Entrez GeneID
8936Protein Accession#
Q92558Gene Name
WASF1
Gene Alias
FLJ31482, KIAA0269, SCAR1, WAVE, WAVE1
Gene Description
WAS protein family, member 1
Omim ID
605035Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000016990|Wiskott-Aldrich syndrome protein family member 1|homology of dictyostelium scar 1|verprolin homology domain-containing protein 1
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Interactome
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Pathway
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