STAT5B (phospho S731) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: EGF (200 ng/mL, 30 mins) treated Raw 264.7 cells, Lane 2: Synthesized peptide treated Raw 264.7 cells with STAT5B (phospho S731) polyclonal antibody (Cat # PAB29588) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma tissue with STAT5B (phospho S731) polyclonal antibody (Cat # PAB29588) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human STAT5B.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S731 of human STAT5B.
Host
Rabbit
Theoretical MW (kDa)
90
Reactivity
Human, Mouse, Rat
Specificity
STAT5B (phospho S731) polyclonal antibody detects endogenous levels of human STAT5B only when phosphorylated at serine 731.
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: EGF (200 ng/mL, 30 mins) treated Raw 264.7 cells, Lane 2: Synthesized peptide treated Raw 264.7 cells with STAT5B (phospho S731) polyclonal antibody (Cat # PAB29588) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast carcinoma tissue with STAT5B (phospho S731) polyclonal antibody (Cat # PAB29588) under 1:50-1:100 dilution (A) or treated with synthesized peptide (B). -
Gene Info — STAT5B
Entrez GeneID
6777Protein Accession#
P51692Gene Name
STAT5B
Gene Alias
STAT5
Gene Description
signal transducer and activator of transcription 5B
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq
Other Designations
transcription factor STAT5B
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Interactome
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Pathway
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Disease
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