BTK polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4 cell lysate, Lane 2: U-251 MG sp cell lysate, Lane 3: A-431 cell lysate, Lane 4: Human liver tissue, Lane 5: Human tonsil tissue with BTK polyclonal antibody (Cat# PAB29308) at 1:100 - 1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymph node tissue with BTK polyclonal antibody (Cat# PAB29308) at 1:200 - 1:500 dilution.
Immunofluorescence
Immunofluorescence staining of U-251 MG cells with BTK polyclonal antibody (Cat# PAB29308) under 1-4 ug/mL working concentration.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human BTK.
Immunogen
Recombinant protein corresponding to amino acids 206-351 of human BTK.
Sequence
PAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4 cell lysate, Lane 2: U-251 MG sp cell lysate, Lane 3: A-431 cell lysate, Lane 4: Human liver tissue, Lane 5: Human tonsil tissue with BTK polyclonal antibody (Cat# PAB29308) at 1:100 - 1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lymph node tissue with BTK polyclonal antibody (Cat# PAB29308) at 1:200 - 1:500 dilution.Immunofluorescence
Immunofluorescence staining of U-251 MG cells with BTK polyclonal antibody (Cat# PAB29308) under 1-4 ug/mL working concentration. -
Gene Info — BTK
Entrez GeneID
695Protein Accession#
Q06187Gene Name
BTK
Gene Alias
AGMX1, AT, ATK, BPK, IMD1, MGC126261, MGC126262, PSCTK1, XLA
Gene Description
Bruton agammaglobulinemia tyrosine kinase
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq
Other Designations
B-cell progenitor kinase|Bruton tyrosine kinase|OTTHUMP00000023676|OTTHUMP00000063593|agammaglobulinemia tyrosine kinase|dominant-negative kinase-deficient Brutons tyrosine kinase|tyrosine-protein kinase BTK
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Interactome
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Pathway
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Disease
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