EIF4A1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF4A1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EIF4A1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EIF4A1 polyclonal antibody (Cat # PAB2836) in K-562 cell line lysates (35 ug/lane). EIF4A1 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of EIF4A1 (arrow) using rabbit EIF4A1 polyclonal antibody (Cat # PAB2836). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EIF4A1 polyclonal antibody (Cat # PAB2836) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — EIF4A1
Entrez GeneID
1973Protein Accession#
NP_001407;P60842Gene Name
EIF4A1
Gene Alias
DDX2A, EIF-4A, EIF4A
Gene Description
eukaryotic translation initiation factor 4A, isoform 1
Omim ID
602641Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000135288|eukaryotic initiation factor 4AI|eukaryotic translation initiation factor 4A isoform 1
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Interactome
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Publication Reference
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PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains.
Suzuki C, Garces RG, Edmonds KA, Hiller S, Hyberts SG, Marintchev A, Wagner G.
PNAS 2008 Mar; 105(9):3274.
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Anti-inflammatory lipid mediator 15d-PGJ2 inhibits translation through inactivation of eIF4A.
Kim WJ, Kim JH, Jang SK.
The EMBO Journal 2007 Nov; 26(24):5020.
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PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains.
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