EIF4A1 recombinant monoclonal antibody, clone 7C3
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate with EIF4A1 recombinant monoclonal antibody, clone 7C3 (Cat # RAB07464).
Immunofluorescence
Immunofluorescent staining of Hela Cells with EIF4A1 recombinant monoclonal antibody, clone 7C3 (Cat # RAB07464). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EIF4A1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human EIF4A1.
Theoretical MW (kDa)
Calculated MW: 40, 4
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate with EIF4A1 recombinant monoclonal antibody, clone 7C3 (Cat # RAB07464).Immunofluorescence
Immunofluorescent staining of Hela Cells with EIF4A1 recombinant monoclonal antibody, clone 7C3 (Cat # RAB07464). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — EIF4A1
Entrez GeneID
1973Protein Accession#
P60842Gene Name
EIF4A1
Gene Alias
DDX2A, EIF-4A, EIF4A
Gene Description
eukaryotic translation initiation factor 4A, isoform 1
Omim ID
602641Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000135288|eukaryotic initiation factor 4AI|eukaryotic translation initiation factor 4A isoform 1
-
Interactome
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com