MAPK3 (phospho T202) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAPK3.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T202 of human MAPK3.
Sequence
F-L-Tp-E-Y
Host
Rabbit
Theoretical MW (kDa)
42, 44
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from MCF7 (Lane 3), 293T(Lane 1 and 2) and Hela (Lane 4) cells using MAPK3 (phospho T202) polyclonal antibody (Cat # PAB25834).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPK3 (phospho T202) polyclonal antibody (Cat # PAB25834).Immunofluorescence
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Gene Info — MAPK3
Entrez GeneID
5595Protein Accession#
P27361Gene Name
MAPK3
Gene Alias
ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
Gene Description
mitogen-activated protein kinase 3
Omim ID
601795Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000174538|OTTHUMP00000174540|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1
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Interactome
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