SIRT6 polyclonal antibody

Catalog # PAB1985

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Size:400 uL
Price: USD $ 329.00
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  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

Western blot analysis of mouse stomach tissue lysate (35 ug/lane) with SIRT6 polyclonal antibody (Cat # PAB1985).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with SIRT6 polyclonal antibody (Cat # PAB1985) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Specification

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of SIRT6.

    Immunogen

    A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SIRT6.

    Host

    Rabbit

    Reactivity

    Human, Mouse

    Form

    Liquid

    Purification

    Protein G purification

    Recommend Usage

    Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Tissue lysate)

    Western blot analysis of mouse stomach tissue lysate (35 ug/lane) with SIRT6 polyclonal antibody (Cat # PAB1985).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with SIRT6 polyclonal antibody (Cat # PAB1985) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Gene Info — SIRT6

    Entrez GeneID

    51548

    Protein Accession#

    NP_057623;Q8N6T7

    Gene Name

    SIRT6

    Gene Alias

    SIR2L6

    Gene Description

    sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)

    Omim ID

    606211

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq

    Other Designations

    sir2-related protein type 6|sirtuin 6|sirtuin type 6

  • Interactome
  • Publication Reference
    • Complete sequencing and characterization of 21,243 full-length human cDNAs.

      Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K

      Nature Genetics 2003 Dec; 36(1):40.

    • Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.

      Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA,

      PNAS 2002 Dec; 99(26):16899.

    • Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins.

      Frye RA.

      Biochemical and Biophysical Research Communications 2000 Jul; 273(2):793.

Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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