MAPK9/MAPK8 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAPK9/MAPK8.
Immunogen
A synthetic peptide corresponding to residues surrounding T183 of human MAPK9/MAPK8.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to MAPK9/MAPK8.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from LoVo cells, using MAPK9/MAPK8 polyclonal antibody (Cat # PAB18484).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using MAPK9/MAPK8 polyclonal antibody (Cat # PAB18484).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of LoVo cells, using MAPK9/MAPK8 polyclonal antibody (Cat # PAB18484).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK8
Entrez GeneID
5599Gene Name
MAPK8
Gene Alias
JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1
Gene Description
mitogen-activated protein kinase 8
Omim ID
601158Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
JNK1 alpha protein kinase|JNK1 beta protein kinase|JUN N-terminal kinase|OTTHUMP00000019552|OTTHUMP00000019555|OTTHUMP00000019556|OTTHUMP00000019558|c-Jun N-terminal kinase 1|mitogen-activated protein kinase 8 isoform JNK1 alpha1|mitogen-activated protein
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Gene Info — MAPK9
Entrez GeneID
5601Gene Name
MAPK9
Gene Alias
JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, p54a, p54aSAPK
Gene Description
mitogen-activated protein kinase 9
Omim ID
602896Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
Jun kinase|MAP kinase 9|c-Jun N-terminal kinase 2|c-Jun kinase 2|mitogen-activated protein kinase 9 isoform JNK2 alpha2|stress-activated protein kinase JNK2
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Interactome
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Pathway
- Adipocytokine signaling pathway
- Adipocytokine signaling pathway
- Colorectal cancer
- Colorectal cancer
- Epithelial cell signaling in Helicobacter pylori infection
- Epithelial cell signaling in Helicobacter pylori infection
- ErbB signaling pathway
- ErbB signaling pathway
- Fc epsilon RI signaling pathway
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Disease
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Publication Reference
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T-cell protein tyrosine phosphatase (Tcptp) is a negative regulator of colony-stimulating factor 1 signaling and macrophage differentiation.
Simoncic PD, Bourdeau A, Lee-Loy A, Rohrschneider LR, Tremblay ML, Stanley ER, McGlade CJ.
Molecular and Cellular Biology 2006 Jun; 26(11):4149.
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The role of protein tyrosine phosphatase 1B in Ras signaling.
Dube N, Cheng A, Tremblay ML.
PNAS 2004 Feb; 101(7):1834.
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Insulin receptor-mediated p62dok tyrosine phosphorylation at residues 362 and 398 plays distinct roles for binding GTPase-activating protein and Nck and is essential for inhibiting insulin-stimulated activation of Ras and Akt.
Wick MJ, Dong LQ, Hu D, Langlais P, Liu F.
The Journal of Biological Chemistry 2001 Nov; 276(46):42843.
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T-cell protein tyrosine phosphatase (Tcptp) is a negative regulator of colony-stimulating factor 1 signaling and macrophage differentiation.
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