PML polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein.
Nuclear extracts (50 ug) from control (Lane 1) and from transfected cells (Lane 2) were analysed by Western blot using PmL polyclonal antibody (Cat # PAB14133).
The antibody was diluted 1 : 750 in TBS-Tween containing 5% milk powder.
The smaller band of approximately 60 kDa might represent the endogenous PmL protein.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of PML polyclonal antibody (Cat # PAB14133), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 6,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PML.
Immunogen
A mixture of synthetic peptides (conjugated with KLH) that corresponding to human PML.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100-1:200)
Western Blot (1:750)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein.
Nuclear extracts (50 ug) from control (Lane 1) and from transfected cells (Lane 2) were analysed by Western blot using PmL polyclonal antibody (Cat # PAB14133).
The antibody was diluted 1 : 750 in TBS-Tween containing 5% milk powder.
The smaller band of approximately 60 kDa might represent the endogenous PmL protein.Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of PML polyclonal antibody (Cat # PAB14133), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 6,000. -
Gene Info — PML
Entrez GeneID
5371Protein Accession#
P29590Gene Name
PML
Gene Alias
MYL, PP8675, RNF71, TRIM19
Gene Description
promyelocytic leukemia
Omim ID
102578Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
promyelocytic leukemia protein|promyelocytic leukemia, inducer of|tripartite motif protein TRIM19
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Interactome
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