PPARG polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
293T cells were analysed by Western blot using PPARG polyclonal antibody (Cat # PAB14095) diluted 1 : 500. Figure shows the result of 293T cells transfected with pNTAP-PPARG (Lane 2) or with an empty vector (Lane 1).
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of PPARG polyclonal antibody (Cat # PAB14095). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 27,600.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PPARG.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human PPARG.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:500)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
293T cells were analysed by Western blot using PPARG polyclonal antibody (Cat # PAB14095) diluted 1 : 500. Figure shows the result of 293T cells transfected with pNTAP-PPARG (Lane 2) or with an empty vector (Lane 1).Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of PPARG polyclonal antibody (Cat # PAB14095). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 27,600. -
Gene Info — PPARG
Entrez GeneID
5468Protein Accession#
P37231Gene Name
PPARG
Gene Alias
CIMT1, NR1C3, PPARG1, PPARG2, PPARgamma
Gene Description
peroxisome proliferator-activated receptor gamma
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000185030|OTTHUMP00000185033|OTTHUMP00000185037|PPAR gamma|nuclear receptor subfamily 1 group C member 3|peroxisome proliferative activated receptor gamma|peroxisome proliferative activated receptor, gamma|peroxisome proliferator activated recept
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Interactome
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Pathway
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Disease
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