PPARG polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using PPARG polyclonal antibody (Cat # PAB11248) shows detection of PPARG protein in a mouse NIH/3T3 whole cell lysate (Lane 1 arrowhead).
Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose.
Primary antibody was used at a 1 : 2,000 dilution in 5% BLOTTO PBS solution.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye.
IgG™800 Conjugated Affinity Purified Goat-anti-Rabbit [H&L] MX10.
Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PPARG.
Immunogen
A synthetic peptide corresponding to N-terminus of human PPARG.
Host
Rabbit
Reactivity
Dog, Human, Macaque, Mouse, Pig
Specificity
This affinity purified antibody is directed against human PPAR gamma isoform 2 protein. No reactivity is expected against other subtypes of PPAR.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000-1:40000)
Western Blot (1:1000-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using PPARG polyclonal antibody (Cat # PAB11248) shows detection of PPARG protein in a mouse NIH/3T3 whole cell lysate (Lane 1 arrowhead).
Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose.
Primary antibody was used at a 1 : 2,000 dilution in 5% BLOTTO PBS solution.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye.
IgG™800 Conjugated Affinity Purified Goat-anti-Rabbit [H&L] MX10.
Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPARG
Entrez GeneID
5468Protein Accession#
P37231;NP_056953Gene Name
PPARG
Gene Alias
CIMT1, NR1C3, PPARG1, PPARG2, PPARgamma
Gene Description
peroxisome proliferator-activated receptor gamma
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000185030|OTTHUMP00000185033|OTTHUMP00000185037|PPAR gamma|nuclear receptor subfamily 1 group C member 3|peroxisome proliferative activated receptor gamma|peroxisome proliferative activated receptor, gamma|peroxisome proliferator activated recept
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Interactome
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Pathway
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Disease
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Publication Reference
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Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes.
De Pascale MC, Bassi AM, Patrone V, Villacorta L, Azzi A, Zingg JM.
Archives of Biochemistry and Biophysics 2006 Mar; 447(2):97.
Application：WB, Human, NCTC 2544 cells.
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Bacillus Calmette-Guerin induces the expression of peroxisome proliferator-activated receptor gamma in bladder cancer cells.
Lodillinsky C, Umerez MS, Jasnis MA, Casabe A, Sandes E, Eijan AM.
International Journal of Molecular Medicine 2006 Feb; 17(2):269.
Application：IF, IHC-P, Human, Mouse, MB49, T24 cells.
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The kinase p38 regulates peroxisome proliferator activated receptor-gamma in human trophoblasts.
Schild RL, Sonnenberg-Hirche CM, Schaiff WT, Bildirici I, Nelson DM, Sadovsky Y.
Placenta 2006 Feb; 27(2-3):191.
Application：WB, Human, Human primary trophoblasts.
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Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes.
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