IL21 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of IL21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with IL21 polyclonal antibody (Cat # PAB12867) at 1 ug /mL .
Immunocytochemistry
Immunocytochemistry of IL21 in K-562 cells with IL21 polyclonal antibody (Cat # PAB12867) at 2 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IL21.
Immunogen
A synthetic peptide corresponding to internal region of human IL21.
Host
Rabbit
Reactivity
Human
Specificity
An approximately 18 KDa band can be detected.
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of IL21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with IL21 polyclonal antibody (Cat # PAB12867) at 1 ug /mL .Immunocytochemistry
Immunocytochemistry of IL21 in K-562 cells with IL21 polyclonal antibody (Cat # PAB12867) at 2 ug/mL . -
Gene Info — IL21
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Pathway
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Disease
- Addison Disease
- Alopecia Areata
- Arthritis
- Asthma
- Autoimmune Diseases
- Bronchiolitis
- Carcinoma
- Celiac Disease
- Colitis
+ View More Disease
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Publication Reference
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Cutting edge: the common gamma-chain is an indispensable subunit of the IL-21 receptor complex.
Asao H, Okuyama C, Kumaki S, Ishii N, Tsuchiya S, Foster D, Sugamura K.
The Journal of Immunology 2001 Jul; 167(1):1.
Application：Stimulated, Recombinant protein.
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Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function.
Parrish-Novak J, Dillon SR, Nelson A, Hammond A, Sprecher C, Gross JA, Johnston J, Madden K, Xu W, West J, Schrader S, Burkhead S, Heipel M, Brandt C, Kuijper JL, Kramer J, Conklin D, Presnell SR, Berry J, Shiota F, Bort S, Hambly K, Mudri S, Clegg C, Moore M, Grant FJ, Lofton-Day C, Gilbert T, Rayond F, Ching A, Yao L, Smith D, Webster P, Whitmore T, Maurer M, Kaushansky K, Holly RD, Foster D.
Nature 2000 Nov; 408(6808):57.
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Cloning of a type I cytokine receptor most related to the IL-2 receptor beta chain.
Ozaki K, Kikly K, Michalovich D, Young PR, Leonard WJ.
PNAS 2000 Oct; 97(21):11439.
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Cutting edge: the common gamma-chain is an indispensable subunit of the IL-21 receptor complex.
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