NCL polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NCL.
Immunogen
A synthetic peptide corresponding to C-terminus of human NCL.
Host
Rabbit
Reactivity
Human, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
Western Blot (1:10000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunoprecipitation
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Gene Info — NCL
Entrez GeneID
4691Gene Name
NCL
Gene Alias
C23, FLJ45706
Gene Description
nucleolin
Omim ID
164035Gene Ontology
HyperlinkGene Summary
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Publication Reference
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Recognition and binding of the human selenocysteine insertion sequence by nucleolin.
Wu R, Shen Q, Newburger PE.
Journal of Cellular Biochemistry 2000 Apr; 77(3):507.
Application：Func, Human, Monkey, COS, HeLa cells.
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Recognition and binding of the human selenocysteine insertion sequence by nucleolin.
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