ATG4C polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot under reducing conditions on rat liver lysate using rabbit ATG4C polyclonal antibody (Cat # PAB0737) at a dilution of 1 : 100.
Western Blot (Cell lysate)
Western blot under reducing conditions on Olfactory cell line (Odora) lysate using rabbit ATG4C polyclonal antibody (Cat # PAB0737) at a dilution of 1 : 100.
Immunofluorescence
Confocal microscopy on immunofluorescently detected ATG4C in HL-60 cell line using rabbit ATG4C polyclonal antibody (Cat # PAB0737) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG4C appear in red. The cells were then counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATG4C.
Immunogen
A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to C-terminus of human ATG4C.
Host
Rabbit
Reactivity
Human, Rat
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:100-1:2000)
Immunofluorescence (1:100-1:2000)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot under reducing conditions on rat liver lysate using rabbit ATG4C polyclonal antibody (Cat # PAB0737) at a dilution of 1 : 100.Western Blot (Cell lysate)
Western blot under reducing conditions on Olfactory cell line (Odora) lysate using rabbit ATG4C polyclonal antibody (Cat # PAB0737) at a dilution of 1 : 100.Immunohistochemistry (Frozen sections)
Immunofluorescence
Confocal microscopy on immunofluorescently detected ATG4C in HL-60 cell line using rabbit ATG4C polyclonal antibody (Cat # PAB0737) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG4C appear in red. The cells were then counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. -
Gene Info — ATG4C
Entrez GeneID
84938Protein Accession#
Q96DT6;Q811C2;109475174Gene Name
ATG4C
Gene Alias
APG4-C, APG4C, AUTL1, AUTL3, FLJ14867
Gene Description
ATG4 autophagy related 4 homolog C (S. cerevisiae)
Omim ID
611339Gene Ontology
HyperlinkGene Summary
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq
Other Designations
APG4 autophagy 4 homolog C|AUT-like 1, cysteine endopeptidase|AUT-like 3 cysteine endopeptidase|OTTHUMP00000010715|autophagin-3|autophagy-related cysteine endopeptidase 3
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Interactome
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Pathway
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