AREG (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human AREG, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 27.2 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human-AREG is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human-AREG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human AREG (P15514, Ser101-Lys187) partial recombinant protein with hFc tag at N-Terminus expressed in HEK293 cells.
Sequence
Ser101-Lys187
Host
Human
Theoretical MW (kDa)
37.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 27.2 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human-AREG is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human-AREG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human AREG, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 27.2 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — AREG
Entrez GeneID
374Protein Accession#
P15514Gene Name
AREG
Gene Alias
AR, CRDGF, MGC13647, SDGF
Gene Description
amphiregulin
Omim ID
104640Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype. [provided by RefSeq
Other Designations
OTTHUMP00000160473|colorectum cell-derived growth factor|schwannoma-derived growth factor
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Interactome
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Pathway
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Disease
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