MAG (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human Siglec-4a, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 3.7 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human Siglec-4a is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Siglec-4a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human MAG (P20916, Gly20-Pro516) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gly20-Pro516
Host
Human
Theoretical MW (kDa)
55.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 3.7 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Siglec-4a is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Siglec-4a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Siglec-4a, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 3.7 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — MAG
Entrez GeneID
4099Protein Accession#
P20916Gene Name
MAG
Gene Alias
GMA, S-MAG, SIGLEC-4A, SIGLEC4A
Gene Description
myelin associated glycoprotein
Omim ID
159460Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
myelin-associated glycoprotein|sialic acid-binding immunoglobulin-like lectin 4A
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Interactome
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Pathway
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Disease
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