IL22RA1 (Human) Recombinant Protein
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Specification
Product Description
Human IL22RA1 (Q8N6P7, His16-Thr228) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
His16-Thr228
Host
Human
Theoretical MW (kDa)
51
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 11.2 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-22R alpha 1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-22R alpha 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-22R alpha 1, hFc Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-IL-22R alpha 1 Antibody, hFc Tag with the EC50 of 11.2 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
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Gene Info — IL22RA1
Entrez GeneID
58985Protein Accession#
Q8N6P7Gene Name
IL22RA1
Gene Alias
CRF2-9, IL22R, IL22R1
Gene Description
interleukin 22 receptor, alpha 1
Omim ID
605457Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the class II cytokine receptor family, and has been shown to be a receptor for interleukin 22 (IL22). IL22 receptor is a protein complex that consists of this protein and interleukin 10 receptor, beta (IL10BR/CRFB4), a subunit also shared by the receptor complex for interleukin 10 (IL10). This gene and interleukin 28 receptor, alpha (IL28RA) form a cytokine receptor gene cluster in the chromosomal region 1p36. [provided by RefSeq
Other Designations
OTTHUMP00000003073
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Interactome
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