Products

Product Description

Human LRRC32/TGFB1 (Q14392(GARP(Y137H))/P01137(Latent TGF beta 1), His20-Leu628(GARP(Y137H))/Leu30-Ser390(Latent TGF beta 1)) partial recombinant protein with His-Avi tag at C-terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Sequence

His20-Leu628(GARP(Y137H));Leu30-Ser390(Latent TGF Beta 1)

Host

Human

Theoretical MW (kDa)

70.3

Form

Lyophilized

Preparation Method

Mammalian cell (HEK293) expression system

Purity

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Endotoxin Level

< 1 EU per 1 ug of protein (determined by LAL method)

Activity

The EC₅₀ was 24.8 ng/mL, messured by ELISA at 5 ug/mL.

Quality Control Testing

SEC-HPLC and Tris-Bis PAGE

SEC-HPLC

The purity of Human GARP (Y137H) &Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC.

Tris-Bis PAGE

Human GARP (Y137H) &Latent TGF Beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Recommend Usage

Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from sterile distilled Water is > 100 ug/mL

Storage Instruction

Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -81°C.
Aliquot to avoid repeated freezing and thawing.

Note

Result of bioactivity analysis