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Product Description

Human TGFB1 (P01137, 30 a.a. - 278 a.a.) partial recombinant protein with His tag at N-terminus expressed in HEK293 cells.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins,Recombinant Human Protein,Recombinant Human Proteins,Human Recombinant Protein,Human Recombinant Proteins,HuPro

Sequence

LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRR

Host

Human

Theoretical MW (kDa)

31.4

Form

Liquid

Preparation Method

Mammalian cell (Expi293, high-yield transient HEK293) expression system

Purity

> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC

Endotoxin Level

< 1 EU per 1 ug of protein (determined by LAL method)

Activity

The EC₅₀ was 0.46 ug/mL, messured by ELISA at 2 ug/mL.

Quality Control Testing

SEC-HPLC and Tris-Bis PAGE

SEC-HPLC

The purity of Human LAP (TGF beta 1) is greater than 90% as determined by SEC-HPLC.

Tris-Bis PAGE

Human LAP (TGF beta 1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Recommend Usage

Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

In PBS pH 7.4

Storage Instruction

Store at -80°C for 12 Month.
Aliquot to avoid repeated freezing and thawing.

Note

Result of bioactivity analysis