TGFB1 (Human) Recombinant Protein
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Specification
Product Description
Human TGFB1 recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Sequence
MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS with polyhistidine tag at the C-terminus.
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 98% as determined by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ ug of protein by the LAL method.
Activity
ED50 < 0.1 ng/mL, measured by the ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The specific activity of recombinant human TGF beta 1 is > 5 x 107 IU/mg. ED50 < 3.2 ng/mL, Measured by the induction of proliferation in MCF-7 cells.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of TGFB1 (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 8.0. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL. In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein.
Storage Instruction
Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C.
Avoid repeated freeze/thaw cycles.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — TGFB1
Entrez GeneID
7040Gene Name
TGFB1
Gene Alias
CED, DPD1, TGFB, TGFbeta
Gene Description
transforming growth factor, beta 1
Gene Ontology
HyperlinkGene Summary
TGFB is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA (MIM 190170) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. TGFB1, TGFB2 (MIM 190220), and TGFB3 (MIM 190230) all function through the same receptor signaling systems.[supplied by OMIM
Other Designations
TGF-beta 1 protein|diaphyseal dysplasia 1, progressive|transforming growth factor-beta 1
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Interactome
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Disease
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