FGF7 (Human) Recombinant protein
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Specification
Product Description
Human FGF7 (P21781) recombinant protein expressed in Escherichia Coli.
Sequence
MCNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT.
Host
Escherichia coli
Theoretical MW (kDa)
18.995
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 96% by SDS-PAGE.
> 96% by RP-HPLC.Activity
The biological activity was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing KGF receptors yielding an ED50 < 10 ng/mL, corresponding to a Specific Activity of 1.0 × 105 IU/mg.
Storage Buffer
Lyophilized from 20mM PB, pH 8.0, 1M NaCl
Storage Instruction
Lyophilized although stable at room temperature for 3 weeks. should be stored desiccated below -20°C. Upon reconstitution should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — FGF7
Entrez GeneID
2252Protein Accession#
P21781Gene Name
FGF7
Gene Alias
HBGF-7, KGF
Gene Description
fibroblast growth factor 7 (keratinocyte growth factor)
Omim ID
148180Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq
Other Designations
fibroblast growth factor 7|heparin-binding growth factor 7|keratinocyte growth factor
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