FGF7 (Human) Recombinant Protein
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Specification
Product Description
Human FGF7 (P21781, 32 a.a. - 194 a.a.) partial recombinant protein expressed in CHO cells.
Sequence
CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT
Host
Mammals
Theoretical MW (kDa)
16 ~ 18
Form
Lyophilized
Preparation Method
_x005F_x000D__x000D_ Mammalian cell (CHO) expression system_x005F_x000D__x000D_
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 1.0 ng/mL, measured in a proliferation assay using 4MBr5 cells.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — FGF7
Entrez GeneID
2252Protein Accession#
P21781Gene Name
FGF7
Gene Alias
HBGF-7, KGF
Gene Description
fibroblast growth factor 7 (keratinocyte growth factor)
Omim ID
148180Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq
Other Designations
fibroblast growth factor 7|heparin-binding growth factor 7|keratinocyte growth factor
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