FGF7 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human FGF7 (P21781) recombinant protein expressed in E.Coli.
Sequence
MCNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT
Host
Escherichia coli
Theoretical MW (kDa)
19
Form
Lyophilized
Purity
>= 90%
Endotoxin Level
<= 1 EUs/ug (Kinetic LAL)
Activity
ED50 <= 60 ng/mL
4MBr-5 cell proliferation
The values provided above are minimum expected values to pass internal requirements.Quality Control Testing
Reducing and Non-Reducing SDS PAGE
Conformation
Monomer
Storage Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.
Storage Instruction
Stored at -20°C to-80°C for 12 month.
After reconstitution with sterile water at 0.1 mg/mL, store at -20°C to -80°C for 3 months, store at 4°C for 1 month.
Aliquot to avoid repeated freezing and thawing.
If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate.Note
Result of activity analysis
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Applications
Western Blot
Functional Study
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Gene Info — FGF7
Entrez GeneID
2252Protein Accession#
P21781Gene Name
FGF7
Gene Alias
HBGF-7, KGF
Gene Description
fibroblast growth factor 7 (keratinocyte growth factor)
Omim ID
148180Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq
Other Designations
fibroblast growth factor 7|heparin-binding growth factor 7|keratinocyte growth factor
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Interactome
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Pathway
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Disease
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