Egf (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Egf (P01132, 977 a.a. - 1029 a.a.) partial-length recombinant protein with N-terminal His-tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELR.
Host
Escherichia coli
Theoretical MW (kDa)
8.6
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Activity
Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 for this effect less or equal to 1ng/mL.
Storage Buffer
EGF protein solution (0.25mg/ml) contains 10% glycerol, 20mM Tris-HCl (pH 8.0), 0.1M NaCl & 2mM DTT.
Storage Instruction
Store at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
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Applications
Functional Study
SDS-PAGE
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Gene Info — Egf
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