Egf (Mouse) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Mouse Egf (P01132, 977 a.a. - 1029 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
MNSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELR
Host
Escherichia coli
Theoretical MW (kDa)
6.2
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/μg of protein by gel clotting method
Activity
ED50 < 0.1 ng/mL, measured by a cell proliferation assay using BALB/c 3T3 cells, corresponding to a specific activity of > 1.0 × 107 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — Egf
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