Egf (Mouse) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
Egf (Mouse) Recombinant Proteinin a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range ≤ 1 ng/mL.
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Specification
Product Description
Mouse Egf (NP_034243, 977 a.a. - 1029a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Host
Mouse
Theoretical MW (kDa)
8.6
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
> 0.2 EU per 1ug of protein (determined by LAL method)
Activity
Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range ≤ 1 ng/ml.
Quality Control Testing
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20mM Tris-HCl, 0.1 M NaCl, pH 8.0 (2 mM DTT, 10% glycerol)
Storage Instruction
Stored at 2°C to 8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — Egf
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