CD3 monoclonal antibody, clone MEM-57 (FITC)
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More Files
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Flow Cytometry
Surface staining of human peripheral blood cells with CD3 monoclonal antibody, clone MEM-57 (FITC) (Cat # MAB4616). Cells in the lymphocyte gate were used for analysis.
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Specification
Product Description
Mouse monoclonal antibody raised against native CD3.
Immunogen
Native purified CD3 from human thymocytes and T lymphocytes.
Host
Mouse
Reactivity
Human
Specificity
This antibody reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
Form
Liquid
Conjugation
FITC
Isotype
IgG2a
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Surface staining of human peripheral blood cells with CD3 monoclonal antibody, clone MEM-57 (FITC) (Cat # MAB4616). Cells in the lymphocyte gate were used for analysis. -
Gene Info — CD3D
Entrez GeneID
915Gene Name
CD3D
Gene Alias
CD3-DELTA, T3D
Gene Description
CD3d molecule, delta (CD3-TCR complex)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq
Other Designations
CD3D antigen, delta polypeptide|CD3d antigen, delta polypeptide (TiT3 complex)|T-cell receptor T3 delta chain|T-cell surface glycoprotein CD3 delta chain
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Gene Info — CD3E
Entrez GeneID
916Gene Name
CD3E
Gene Alias
FLJ18683, T3E, TCRE
Gene Description
CD3e molecule, epsilon (CD3-TCR complex)
Omim ID
186830Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq
Other Designations
CD3-epsilon|CD3E antigen, epsilon polypeptide|CD3e antigen, epsilon polypeptide (TiT3 complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain|T-cell surface glycoprotein CD3 epsilon chain
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Gene Info — CD3G
Entrez GeneID
917Gene Name
CD3G
Gene Alias
CD3-GAMMA, FLJ17620, FLJ17664, FLJ79544, FLJ94613, MGC138597, T3G
Gene Description
CD3g molecule, gamma (CD3-TCR complex)
Omim ID
186740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq
Other Designations
CD3G antigen, gamma polypeptide|CD3g antigen, gamma polypeptide (TiT3 complex)|T-cell antigen receptor complex, gamma subunit of T3|T-cell receptor T3 gamma chain|T-cell surface glycoprotein CD3 gamma chain
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Interactome
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Pathway
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Disease
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Publication Reference
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T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity.
Alarcon B, Swamy M, van Santen HM, Schamel WW.
EMBO Reports 2006 May; 7(5):490.
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Therapeutic in vivo use of the A1-CD3 monoclonal antibody.
I Hilgert , F Franĕk, I Stefanová, J Kaslík, J Jirka, H Kristofová, P Rossmann, J Soucek, V Horejsi.
Transplantation 1993 Feb; 55(2):435.
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Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I.
Folia Biol (Praha) 1988 Jan; 34(1):23.
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T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity.
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