CD3E recombinant monoclonal antibody, clone R02-1B5
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More Files
- More Functions
Western Blot
Western blot analysis of CD3 epsilon in Jurkat lysates using human CD3 epsilon recombinant monoclonal antibody, clone R02-1B5 (Cat # RAB01525).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human tonsil with CD3 epsilon recombinant monoclonal antibody, clone R02-1B5 (Cat # RAB01525).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human CD3 epsilon.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD3 epsilon
Theoretical MW (kDa)
Calculated MW: 23 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1/100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of CD3 epsilon in Jurkat lysates using human CD3 epsilon recombinant monoclonal antibody, clone R02-1B5 (Cat # RAB01525).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human tonsil with CD3 epsilon recombinant monoclonal antibody, clone R02-1B5 (Cat # RAB01525).Immunoprecipitation
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Gene Info — CD3E
Entrez GeneID
916Protein Accession#
P07766Gene Name
CD3E
Gene Alias
FLJ18683, T3E, TCRE
Gene Description
CD3e molecule, epsilon (CD3-TCR complex)
Omim ID
186830Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq
Other Designations
CD3-epsilon|CD3E antigen, epsilon polypeptide|CD3e antigen, epsilon polypeptide (TiT3 complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain|T-cell surface glycoprotein CD3 epsilon chain
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Interactome
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Pathway
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Disease
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