Anti-Cetuximab monoclonal antibody, clone 69H10
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Cetuximab monoclonal antibody, clone 69H10 (Cat # MAB23085) with Cetuximab. Coating antigen: Cetuximab, 1 ug/mL. Anti-Cetuximab monoclonal antibody, clone 69H10 dilutions start from 1000 ng/mL. EC50 = 17.67 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Sandwich ELISA
The Sandwich ELISA assay is developed by using Cetuximab monoclonal antibody, clone 69H10 (Cat # MAB23085) and Anti-Cetuximab monoclonal antibody, clone 90G3 (Cat # MAB23086) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Cetuximab monoclonal antibody, clone 90G3 was labeled with Biotin. The sensitivity of detecting Cetuximab is up to 40 pg/mL.
Blocking
Anti-Cetuximab monoclonal antibody, clone 69H10 (Cat # MAB23085) blocks Cetuximab binding with EGFR protein. Coating antigen: EGFR, 0.5 ug/mL. Cetuximab final concentration: 20 ng/mL. The antibody dilutions start from 10 ug/mL. IC50 = 39.3 ng/mL.
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Specification
Product Description
Rabbit monoclonal antibody raised against Cetuximab.
Target gene is EGFR.Immunogen
Cetuximab.
Host
Rabbit
Specificity
The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody : Anti-Cetuximab monoclonal antibody, clone 90G3 (Cat # MAB23086).
Form
Lyophilized
Preparation Method
The antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Cetuximab.
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Blocking (10-50 ug/mL)
ELISA (0.01-0.1 ug/mL (Direct/Indirect/Inhibitory))
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.039 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Cetuximab monoclonal antibody, clone 69H10 (Cat # MAB23085) with Cetuximab. Coating antigen: Cetuximab, 1 ug/mL. Anti-Cetuximab monoclonal antibody, clone 69H10 dilutions start from 1000 ng/mL. EC50 = 17.67 ng/mL. While the antibody does not recognize the human IgG Fc fragment (data not shown).Sandwich ELISA
The Sandwich ELISA assay is developed by using Cetuximab monoclonal antibody, clone 69H10 (Cat # MAB23085) and Anti-Cetuximab monoclonal antibody, clone 90G3 (Cat # MAB23086) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Cetuximab monoclonal antibody, clone 90G3 was labeled with Biotin. The sensitivity of detecting Cetuximab is up to 40 pg/mL.Blocking
Anti-Cetuximab monoclonal antibody, clone 69H10 (Cat # MAB23085) blocks Cetuximab binding with EGFR protein. Coating antigen: EGFR, 0.5 ug/mL. Cetuximab final concentration: 20 ng/mL. The antibody dilutions start from 10 ug/mL. IC50 = 39.3 ng/mL. -
Gene Info — EGFR
Entrez GeneID
1956Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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