YWHAG monoclonal antibody, clone HS23
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of YWHAG in HeLa cell lysate (Lane1), and bengamide treated lysates (Lane 2 and 4, for 8h and 24h, respectively) with YWHAG monoclonal antibody, clone HS23 (Cat # MAB2288).
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Specification
Product Description
Mouse monoclonal antibody raised against YWHAG.
Immunogen
N-terminus of human YWHAG.
Host
Mouse
Reactivity
Bovine, Chicken, Human, Mouse, Rat, Zebra fish
Specificity
This antibody is specific to human 14-3-3 gamma.
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against YWHAG.
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.02% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of YWHAG in HeLa cell lysate (Lane1), and bengamide treated lysates (Lane 2 and 4, for 8h and 24h, respectively) with YWHAG monoclonal antibody, clone HS23 (Cat # MAB2288).Immunocytochemistry
Immunofluorescence
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Gene Info — YWHAG
Entrez GeneID
7532Gene Name
YWHAG
Gene Alias
14-3-3GAMMA
Gene Description
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
Omim ID
605356Gene Ontology
HyperlinkGene Summary
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq
Other Designations
14-3-3 gamma
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Interactome
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Pathway
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Publication Reference
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Elucidation of the function of type 1 human methionine aminopeptidase during cell cycle progression.
Hu X, Addlagatta A, Lu J, Matthews BW, Liu JO.
PNAS 2006 Nov; 103(48):18148.
Application：WB, Human, HeLa cells.
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Elucidation of the function of type 1 human methionine aminopeptidase during cell cycle progression.
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