EEF2 monoclonal antibody, clone ADBH-5
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) A431, (2) NIH/3T3 cell lysates.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic protein of human EEF2.
Immunogen
A synthetic peptide corresponding to human EEF2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with human, mouse, rat EEF2, in native form and recombinant. Superfamily members of EEF2 are not reactive to antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
Immunofluorescence (1:50-200)
Immunocytochemistry (1:50-200)
Immunoprecipitation (1:20)
Western Blot (1:1000-5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) A431, (2) NIH/3T3 cell lysates.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — EEF2
Entrez GeneID
1938Protein Accession#
P13639Gene Name
EEF2
Gene Alias
EEF-2, EF2
Gene Description
eukaryotic translation elongation factor 2
Omim ID
130610Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq
Other Designations
polypeptidyl-tRNA translocase
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Interactome
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Disease
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