EEF2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EEF2.
Immunogen
A synthetic peptide corresponding to human EEF2.
Host
Rabbit
Theoretical MW (kDa)
100
Reactivity
Human, Mouse, Rat
Specificity
EEF2 polyclonal antibody detects endogenous levels of EEF2 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EEF2 polyclonal antibody (Cat # PAB27058) in extracts from NIH/3T3 cells treated with serum 10% 30'.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of EEF2 polyclonal antibody (Cat # PAB27058) in paraffin-embedded human breast carcinoma tissue.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — EEF2
Entrez GeneID
1938Protein Accession#
P13639Gene Name
EEF2
Gene Alias
EEF-2, EF2
Gene Description
eukaryotic translation elongation factor 2
Omim ID
130610Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq
Other Designations
polypeptidyl-tRNA translocase
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Interactome
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