Histone H3 (K4) monoclonal antibody, clone RM186
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More Files
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Western Blot
Western blot analysis of Lane 1: acid extracts of HeLa cell treated with sodium butyrate (HeLa+NaBu), Lane 2: acid extracts of HeLa cell treated with Nocodazole (HeLa+Noc), Lane 3: acid extracts of HeLa cell untreated and Lane 4: recombinant Histone H3.1 with Histone H3 (K4) monoclonal antibody, clone RM186 (Cat # MAB12789) at 1 ug/mL working concentration, showed a band of Histone H3 with unmodified Lysine 4.
Immunocytochemistry
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Histone H3 (K4) monoclonal antibody, clone RM186 (Cat# MAB12789) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Histone H3 (K4) monoclonal antibody, clone RM186 (Cat# MAB12789) specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono- methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against of human histone H3 (K4).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to residues surrounding K4 at N-terminus region of human Histone H3.
Sequence
N/A
Specificity
This antibody recognizes Histone H3 that is unmodified at Lys 4 but does not recognize acetylated or methylated Lys 4. The antibody binding specificity allows for modifications of Arg 2, Thr 3, and/or other modifications in Histone H3.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (0.5 ug/mL-1 ug/mL)
Western Blot (1 ug/mL-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: acid extracts of HeLa cell treated with sodium butyrate (HeLa+NaBu), Lane 2: acid extracts of HeLa cell treated with Nocodazole (HeLa+Noc), Lane 3: acid extracts of HeLa cell untreated and Lane 4: recombinant Histone H3.1 with Histone H3 (K4) monoclonal antibody, clone RM186 (Cat # MAB12789) at 1 ug/mL working concentration, showed a band of Histone H3 with unmodified Lysine 4.Immunocytochemistry
Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Histone H3 (K4) monoclonal antibody, clone RM186 (Cat# MAB12789) (red). Actin filaments have been labeled with fluorescein phalloidin (green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HIST1H3A
Entrez GeneID
8350Protein Accession#
Q16695Gene Name
HIST1H3A
Gene Alias
H3/A, H3FA
Gene Description
histone cluster 1, H3a
Omim ID
602810Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member A|histone 1, H3a
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