CCNB1 monoclonal antibody, clone 5G6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CCNB1 monoclonal antibody, clone 5G6 (Cat # MAB10520) against HeLa (1) and PC-12 (2) cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using CCNB1 monoclonal antibody, clone 5G6 (Cat # MAB10520) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of HeLa cells using CCNB1 monoclonal antibody, clone 5G6 (Cat # MAB10520) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CCNB1.
Immunogen
Recombinant protein corresponding to human CCNB1.
Host
Mouse
Theoretical MW (kDa)
60
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CCNB1 monoclonal antibody, clone 5G6 (Cat # MAB10520) against HeLa (1) and PC-12 (2) cell lysate.Immunofluorescence
Immunofluorescence analysis of HeLa cells using CCNB1 monoclonal antibody, clone 5G6 (Cat # MAB10520) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using CCNB1 monoclonal antibody, clone 5G6 (Cat # MAB10520) (green) and negative control (purple). -
Gene Info — CCNB1
Entrez GeneID
891Gene Name
CCNB1
Gene Alias
CCNB
Gene Description
cyclin B1
Omim ID
123836Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq
Other Designations
G2/mitotic-specific cyclin B1
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Interactome
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Pathway
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Disease
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