CCNB1 recombinant monoclonal antibody, clone R01-7H7
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More Files
- More Functions
Western Blot
Western blot analysis of Cyclin B1 in Jurkat lysates using human Cyclin B1 recombinant monoclonal antibody, clone R01-7H7 (Cat # RAB01663).
Western Blot
Western blot analysis of Lane 1: K562 and Lane 2: Hela lysates with Cyclin B1 recombinant monoclonal antibody, clone R01-7H7 (Cat # RAB01663).
Immunocytochemistry
Immunocytochemical staining of Hela with Cyclin B1 recombinant monoclonal antibody, clone R01-7H7 (Cat # RAB01663). (A) Cyclin B1 (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Cyclin B1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Cyclin B1
Theoretical MW (kDa)
Calculated MW: 48 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Cyclin B1 in Jurkat lysates using human Cyclin B1 recombinant monoclonal antibody, clone R01-7H7 (Cat # RAB01663).Western Blot
Western blot analysis of Lane 1: K562 and Lane 2: Hela lysates with Cyclin B1 recombinant monoclonal antibody, clone R01-7H7 (Cat # RAB01663).Immunocytochemistry
Immunocytochemical staining of Hela with Cyclin B1 recombinant monoclonal antibody, clone R01-7H7 (Cat # RAB01663). (A) Cyclin B1 (green) and (B) DAPI (blue).Immunoprecipitation
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Gene Info — CCNB1
Entrez GeneID
891Protein Accession#
P14635Gene Name
CCNB1
Gene Alias
CCNB
Gene Description
cyclin B1
Omim ID
123836Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq
Other Designations
G2/mitotic-specific cyclin B1
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Interactome
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Pathway
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Disease
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