TBK1 monoclonal antibody, clone 108A429
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of TBK1 in (A) human Daudi cell lysate and (B) mouse RAW cell lysate using TBK1 monoclonal antibody, clone 108A429 (Cat # MAB0053) at 2 ug/mL .
Western Blot (Transfected lysate)
Western blot analysis of TBK1. Using TBK1 monoclonal antibody, clone 108A429 (Cat # MAB0053) 2 ug/mL dilution against 10 ug/lane of lysate from 293 cells transfected with human TBK1 cDNA.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of TBK1.
Immunogen
A synthetic peptide corresponding to amino acids 563-577 of human TBK1.
Sequence
YYQFKKDKAERRLAYC
Host
Mouse
Reactivity
Bovine, Dog, Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of TBK1 in (A) human Daudi cell lysate and (B) mouse RAW cell lysate using TBK1 monoclonal antibody, clone 108A429 (Cat # MAB0053) at 2 ug/mL .Western Blot (Transfected lysate)
Western blot analysis of TBK1. Using TBK1 monoclonal antibody, clone 108A429 (Cat # MAB0053) 2 ug/mL dilution against 10 ug/lane of lysate from 293 cells transfected with human TBK1 cDNA. -
Gene Info — TBK1
Entrez GeneID
29110Protein Accession#
NP_037386.1Gene Name
TBK1
Gene Alias
FLJ11330, NAK, T2K
Gene Description
TANK-binding kinase 1
Omim ID
604834Gene Ontology
HyperlinkGene Summary
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK. [provided by RefSeq
Other Designations
NF-kB-activating kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Glucocorticoids inhibit IRF3 phosphorylation in response to Toll-like receptor-3 and -4 by targeting TBK1 activation.
McCoy CE, Carpenter S, Palsson-McDermott EM, Gearing LJ, O'Neill LA.
The Journal of Biological Chemistry 2008 Mar; 283(21):14277.
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Interferon regulatory factor 3-dependent responses to lipopolysaccharide are selectively blunted in cord blood cells.
Aksoy E, Albarani V, Nguyen M, Laes JF, Ruelle JL, De Wit D, Willems F, Goldman M, Goriely S.
Blood 2007 Apr; 109(7):2887.
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Regulation of IkappaB kinase epsilon expression by the androgen receptor and the nuclear factor-kappaB transcription factor in prostate cancer.
Peant B, Diallo JS, Lessard L, Delvoye N, Le Page C, Saad F, Mes-Masson AM.
Molecular Cancer Research 2007 Jan; 5(1):87.
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IKK-i signals through IRF3 and NFkappaB to mediate the production of inflammatory cytokines.
Sankar S, Chan H, Romanow WJ, Li J, Bates RJ.
Cellular Signalling 2005 Sep; 18(7):982.
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Gene expression and antiviral activity of alpha/beta interferons and interleukin-29 in virus-infected human myeloid dendritic cells.
Osterlund P, Veckman V, Siren J, Klucher KM, Hiscott J, Matikainen S, Julkunen I.
Journal of Virology 2005 Aug; 79(15):9608.
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Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I.
Sumpter R Jr, Loo YM, Foy E, Li K, Yoneyama M, Fujita T, Lemon SM, Gale M Jr.
Journal of Virology 2005 Mar; 79(5):2689.
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IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway.
Fitzgerald KA, McWhirter SM, Faia KL, Rowe DC, Latz E, Golenbock DT, Coyle AJ, Liao SM, Maniatis T.
Nature Immunology 2003 May; 4(5):491.
Application：IP, WB-Tr, Human, HEK 293T cells.
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Glucocorticoids inhibit IRF3 phosphorylation in response to Toll-like receptor-3 and -4 by targeting TBK1 activation.
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