ERN1 monoclonal antibody (M02), clone 8D12
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More Files
- More Functions
Western Blot (Cell lysate)
ERN1 monoclonal antibody (M02), clone 8D12. Western Blot analysis of ERN1 expression in LNCaP.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ERN1 is approximately 0.03ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TRAF2 and ERN1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ERN1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to ERN1 on HeLa cell. [antibody concentration 25 ug/ml]
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ERN1.
Immunogen
ERN1 (AAC25991, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (87)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ERN1 monoclonal antibody (M02), clone 8D12. Western Blot analysis of ERN1 expression in LNCaP.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ERN1 is approximately 0.03ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TRAF2 and ERN1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ERN1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to ERN1 on HeLa cell. [antibody concentration 25 ug/ml] -
Gene Info — ERN1
Entrez GeneID
2081GeneBank Accession#
AF059198Protein Accession#
AAC25991Gene Name
ERN1
Gene Alias
FLJ30999, IRE1, IRE1P, MGC163277, MGC163279
Gene Description
endoplasmic reticulum to nucleus signaling 1
Omim ID
604033Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq
Other Designations
ER to nucleus signalling 1|endoplasmic reticulum to nucleus signalling 1|inositol-requiring 1|protein kinase/endoribonuclease
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Interactome
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Disease
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