ERN1 (phospho S724) recombinant monoclonal antibody, clone 1F12
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More Files
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Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 100 nM/60 mins), Lane 2: 293 whole cell lysate (not treated), Lane 3: A549 whole cell lysate (treated with Calyculin A 100 nM/30 mins), Lane 4: A549 whole cell lysate (not treated), Lane 5: Hela whole cell lysate (treated with EGF 100 ng/mL/20 mins), Lane 6: Hela whole cell lysate (not treated) with ERN1 (phospho S724) recombinant monoclonal antibody, clone 1F12 (Cat # RAB04244).
Immunofluorescence
Immunofluorescent staining of Hela cells with ERN1 (phospho S724) recombinant monoclonal antibody, clone 1F12 (Cat # RAB04244) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ERN1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S724 of human ERN1.
Theoretical MW (kDa)
Calculated MW: 110 k
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 100 nM/60 mins), Lane 2: 293 whole cell lysate (not treated), Lane 3: A549 whole cell lysate (treated with Calyculin A 100 nM/30 mins), Lane 4: A549 whole cell lysate (not treated), Lane 5: Hela whole cell lysate (treated with EGF 100 ng/mL/20 mins), Lane 6: Hela whole cell lysate (not treated) with ERN1 (phospho S724) recombinant monoclonal antibody, clone 1F12 (Cat # RAB04244).Immunofluorescence
Immunofluorescent staining of Hela cells with ERN1 (phospho S724) recombinant monoclonal antibody, clone 1F12 (Cat # RAB04244) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ERN1
Entrez GeneID
2081Protein Accession#
O75460Gene Name
ERN1
Gene Alias
FLJ30999, IRE1, IRE1P, MGC163277, MGC163279
Gene Description
endoplasmic reticulum to nucleus signaling 1
Omim ID
604033Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq
Other Designations
ER to nucleus signalling 1|endoplasmic reticulum to nucleus signalling 1|inositol-requiring 1|protein kinase/endoribonuclease
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Interactome
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Disease
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