VHH antibody, also known as Camelid single domain antibody (sdAb), is the recombinant variable domain derived from camelid heavy-chain-only antibody. This configuration confers a number of unique properties. The small size and high stability of VHH antibody make it a promising tool for disease diagnosis and treatment. VHH antibody can penetrate tissue more easily and target difficult epitopes hidden in the protein structure. Additionally, VHH antibody expresses well in many host systems, making it cost-effective for large-scale manufacturing.

Abnova offers the NanoAb™ service for the production of target-specific VHH antibodies from the camelid VHH library. Using advanced camelid VHH library screening technology, we screen the library and isolate the VHH clones for your required antigens. Positive clones are sequenced and validated by ELISA. The selected clones will then be introduced into the HEK293 expression vector or E. coli expression vector for transient and scalable VHH antibody production, suitable for many downstream applications.

Custom_NanoAb.pdf

NanoAb™ Workflow
NanoAb_workflow.jpg (463 KB)
 
 

Specification

 

Naïve Camelid VHH Library

Immune Library

Library Size

1.2 x 1012

1.2 x 1013

Required Screening Antigen Amount

0.6 mg

3.5 mg

Lead Time

8-10 weeks

22-24 weeks

Deliverables
  1. Phage validation through phage ELISA data
  2. Up to 5 different VHH sequences
  3. Up to 1 purified VHH, approximately 0.2 mg per VHH
  1. cDNA 150ug
  2. VHH library 3-5ml
  3. Phage validation through phage ELISA data
  4. Up to 8 different VHH sequences
  5. Up to 1 purified VHH, approximately 0.2 mg per VHH
 

Advantages

  • Small Size

  • Superior Stability

  • Low Immunogenicity

  • High Affinity and Specificity

  • Fast Blood Clearance

  • Versatile Production

  • Easy Multimerization (Multivalency/Multiparatopicity/Multispecificity)

  • Easy Conjugation

  • Robustness in Bioengineering

  • Applications Across Fields
 
 

VHH Antibody vs. Conventional Mab and Pab

 

 VHH Antibody

Mab

Pab

 Size

12 - 15 kD

Approximately 150 kD

Approximately 150 kD

Required Antigen Amount

≦1 mg

≦4 mg

≦4 mg

 Method

Camelid VHH library screening

Hybridoma technology

Harvested in antiserum

Stability

High stability to heat, pH and denaturing agents

Thermal instability

Biological instability

Lot-to-Lot Consistency

Very high

High

Low

 Lead Time

8-24 weeks

16-24 weeks

4-12 weeks
 

Examples

Customized CD8A VHH-hIgG1 Humanized Monoclonal Antibodies

Western Blot

NanoAb_CD8A_WB.jpg (63 KB)
 
 

Flow Cytometry

NanoAb_CD8A_flow.jpg (223 KB)
 

For any inquiry, please contact : OEM@abnova.com

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